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Image Search Results
Journal: Journal of the American Chemical Society
Article Title: Stable Cu(II) and Cu(I) Mononuclear Intermediates in the Assembly of the CuA center of Thermus thermophilus Cytochrome Oxidase
doi: 10.1021/ja307276z
Figure Lengend Snippet: Stopped-flow kinetic analysis of the reaction of the bis-thiol apo M160SeM CuA with aqueous Cu(II) sulfate. (a) Transient spectra collected at increasing time intervals color coded as follows: red (0.04 – 84 ms), green (1 – 1.64 s), blue (2.84 – 9.64 s), purple (final products above 10 s). (b) Fit to the absorbance versus time data collected at 462 nm. (c) Absorption spectra of colored species (including intermediates) derived from a global kinetic analysis of data at all wavelengths, using the Pro-K II software package. (d) Time dependence of the formation and decay of colored species during the reaction. Fits, spectra of intermediates, and formation-decay curves were derived using the mechanism and rate constants listed in Table 2. Concentrations were apo protein, 170 μM and Cu(II) 500 μM
Article Snippet: Kinetic data were analyzed using the program DYNAFIT 34 and by the
Techniques: Derivative Assay, Software